ANTIPLASMODIUM ACTIVITIES OF KELUWIH (Artocarpus camansi) METHANOL LEAF EXTRACTS IN THE MENCIT (Mus musculus) Balb / c INFECTED WITH Plasmodium berghei

Dmitry Arditya Harsya Priangga, Dwi Soelistya Dyah Jekti, Yayuk Andayani

Abstract


Antiplasmodium activity test of methanol extract of leaves of kelwih (Artocarpus camansi) was carried out in vivo in mice (Mus musculus) Balb / c infected with Plasmodium berghei. The purpose of this study was to determine the effective dose of methanol extract of leaves of kelwih as antiplasmodium. Antiplasmodium activity test was carried out in vivo in Balb / c mice that had been infected with Plasmodium berghei. Parameters observed were parasite growth, and parasite inhibition. After the administration of methanol extract of leaves of kelwih and as many as 30 mice were grouped into 5 treatment groups which were given extract doses of 1, 25, 50, 75, and 100 mg / kg BB and one negative control group (without the administration of methanol extract of kelwih leaves). Observations were carried out for 7 days, starting from day 0 (before treatment), 4 days during treatment and 2 days after treatment. The data obtained were analyzed statistically ANOVA using the Kruskal-Wallis H test and continued with Mann-Whitney U test. The results showed that the lowest parasite growth (1.636%) was obtained from the group dose of 100 mg / kg BB, the largest parasite inhibition (72.832% ) also produced by a group dose of 100 mg / kg BB. An extract is said to have positive antimalarial activity if it can reduce parasitemia by 30% or more. A dose of 100 mg / kg body weight produces parasitic growth values, and parasite inhibition is significant to the control (p <0.05). So, from the explanation above, it can be concluded that giving a dose of 100 mg / kg BB of methanol extract of leaves of kelwih has potential activity as an antimalarial.

Keywords


Antiplasmodium, Artocarpus camansi, Plasmodium berghei

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References


Afrilyana M. 2012. Analisis Toksikoorganologi dan Fungsi Organ Hati dan Ginjal Mencit (Mus musculus) Pasca Pemberian Ekstrak Metanol Daun Keluwih (Artocarpus camansi). Thesis S2. Universitas Mataram.

Akuodor GC, Idris, Usman M, Anyalewechi N, Odo E, Ugwu CT, Akpan JL, Gwotmut MD, Osunkwo UA. 2010. In Vivo Antimalaria Activity of Ethanolic Leaf Ekstract of Verbena Hastata Against Plasmodium Berghei in Mice. Journal of Herbal Medicine and Toxicology. 4 (2) : 17-23.

Andayani Y, Jekti DS, Hakim A. 2009. Aktivitas Antimalaria dan Analisis Metabolit Sekunder dari Ekstrak Buah, Daun, dan Kulit Batang Artocarpus camansi.Laporan penelitian tidak dipublikasikan.

Batista R, Silva AdJ. Jr., and de Oliveria AB. 2009. Plant-derived Antimalarial Agent: New Leads and Efficient Phytomedicine. Part II.Non-Alkaloidal Natural Products. Molecules. 14 : 3037-3072

Boonphong, S., Baramee, A., Kittakoop, P, Puangsombat, P., (2007). Antitubercular and Antiplasmodial Prenylated Flavones from The Root of Artocarpus altilis. Chiang Mai J. Sci, 34(3) : 339-343.

Egan TJ, Combrinck JM, Egan J, Hearne GR, Marquess HM, Ntenteni S, Sewel BT, Smith PJ, Taylor D, Schalkwyk DA, Walden JC. 2002. Fate of Haem Iron in The Malaria Parasite Plasmodium falciparum. J Biochem. 365, 343-347.

Fuad A, Widyawaruyanti A, Radjaram A, Santosa M H, dan Miatmoko A. 2010. Pengembangan Produk Obat Herbal Terstandar Sebagai Obat Antimalaria dari Ekstrak Kulit Batang Cempedak (Artocarpus champeden SPRENG). http://penelitian.unair.ac.id/dosen_detail_756. Diakses pada 11-2-2012.

Kabiru YA, Okolie NL, Muhammad HL, Ogbadoyi EO. 2012. Preliminary Studied on The Antiplasmodial Potential of Aqueous and Methanol Extract of Eucalyptus camadulensis Leaf. Asian Pacific Journal of Tropical Disease. S809 – S814.

Madara AA, Tijani AY, Nandi EP. 2012. Anti-Plamodial Activity of Root Bark Extract of Piliostigma thonningii Schum.(Caesalpiniacea) in Mice Infected with Plasmodium bergheii berghei NK 65. Rep Opinion. 4(4):62-67.

Nisa K. 2011. Aktivitas Antimalaria Secara In Vitro dari Ekstrak Metanol Daun dan Ekstrak Metanol Kulit Batang Artocarpus camansi (Keluwih) pada Plasmodium falsiparum. Thesis S2.Universitas Mataram.

Othuke OB, Uwakwe AA, Monago CC. 2012. Antiplasmodial Activity of Metanolic Stem Bark Ekstract of Anthocleista grandiflora in Mice.Internasional Journal of Applied Science and Technology. 2 (4): 142-148.

Pratiwi, Harapini M, dan Chairul. 2007. Uji Aktivitas Antimalaria Secara In Vivo Ekstrak Ki Pahit (Picrasma javanica) pada Mencit yang Diinfeksi Plasmodium berghei. Journal Biodiversitas. 8(2) : 111-113.

Suryani D. 2011. Pengaruh Lama Pemberian Ekstrak Teripang Pasir (Holothuria scabra) Terhadap Tingkat Parasitemia Plasmodium berghei Pada Mencit Balb/c. Thesis S2. Universitas Mataram.

Syafruddin D. 2009. Dasar Molekul Resistansi Parasit Terhadap Obat Antimalaria dalam Malaria dari Molekular ke Klinis oleh Harijanto dkk. Penerbit buku kedokteran EGC. Jakarta.

World Health Organization. 2013. Malaria. http://www.who.int/mediacentre/ factsheets/fs094/en/. (akses 23 Maret 2013).




DOI: https://doi.org/10.33394/j-ps.v1i2.1016

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J-PS (Prisma Sains: Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram) p-ISSN (print) 2338-4530, e-ISSN (online) 2540-7899 is licensed under a Creative Commons Attribution 4.0 International License.

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